Journal: Scientific Reports

Article Title: Effects of an early life diet containing large phospholipid-coated lipid globules on hepatic lipid metabolism in mice

doi: 10.1038/s41598-020-72777-y

Figure Lengend Snippet: Body weight, fat mass and lean mass gain during eIMF or cIMF feeding. Plasma lipids, adipokines, cytokines and bile acids at PN42. Body weight ( A ), fat and lean mass ( B ) are expressed in absolute weights. Plasma lipids at PN21 (weaning) and at PN42 ( C ). All other parameters were measured at PN42. Liver, epididymal (epi) and perirenal (peri) fat pads weights obtained at dissection at PN42 are expressed absolutely ( D ). Plasma adipokines ( E , F ), glucostatic hormones ( G ) and cytokines ( H , all 4 h fasting at PN42) are expressed as absolute concentration. Plasma bile acid species are expressed as a percentage ( I , PN42). TG triglycerides, TC total cholesterol, FC free cholesterol, CE cholesterol ester, NEFA non-esterified fatty acids, Phos phospholipids, MCP-1 monocyte chemoattractant protein-1, TNFα tumor necrosis factor alpha, IL-1b/2/5/6/10 interleukin 1b/2/5/6/10, IFNg interferon gamma, CXCL-1 CXC chemokine ligand 1, (T-) (L) CA (tauro-) (litho) cholic acid, (T/G-) (U/C/H) DCA (tauro-) (urso/cheno/hyo) deoxycholic acid, (T)A/B-MCA (tauro-) α/β-muricholic acid. ( A – I ) n = 12–16; ( A , B ) Median ± interquartile range. ( C – I ) Tukey boxplots and scatter plots. Exact two-sided Mann–Whitney U test **p < 0.01, *p < 0.05.

Article Snippet: Plasma was analyzed using commercially available kits for triglycerides (Roche, 11877771216), total cholesterol (Roche, 11491458216), free cholesterol (Spinreact, 41035), NEFA (Sopachem, 157819910935), and phospholipids (Sopachem, 157419910930).

Techniques: Clinical Proteomics, Dissection, Concentration Assay, MANN-WHITNEY

Journal: Scientific Reports

Article Title: Effects of an early life diet containing large phospholipid-coated lipid globules on hepatic lipid metabolism in mice

doi: 10.1038/s41598-020-72777-y

Figure Lengend Snippet: Calculated nutrient composition of the diets.

Article Snippet: Plasma was analyzed using commercially available kits for triglycerides (Roche, 11877771216), total cholesterol (Roche, 11491458216), free cholesterol (Spinreact, 41035), NEFA (Sopachem, 157819910935), and phospholipids (Sopachem, 157419910930).

Techniques: Maltodextrin, Starch

Journal: PLoS ONE

Article Title: Spontaneous liver disease in wild-type C57BL/6JOlaHsd mice fed semisynthetic diet

doi: 10.1371/journal.pone.0232069

Figure Lengend Snippet: Hepatic TG and total cholesterol (μmol/g) and protein (mg/g) are expressed as absolute concentrations (A). Fasting plasma ASAT (GOT) and ALAT (GPT) are expressed as units/L (B). Fasting triglycerides (TG), total (TC), free (FC), esterified (CE) cholesterol, and non-esterified fatty acids (NEFA, E) are expressed as absolute concentrations. Hepatic gene expression was corrected for 36b4 and shown as fold-change versus NL (D, G-H). The hepatic FA profile is shown as the fold-change of absolute values versus NL (E). The hepatic DHA status was calculated as the molar ratio 22:5ω6/22:6ω3 (F). Abbreviations used: ASAT/ALAT: aspartate/alanine aminotransferase. 14:0 myristic acid, 16:0 palmitic acid, 18:0 stearic acid, 20:0 arachidic acid, 22:0 behenic acid, 24:0 lignoceric acid, 18:3ω3 α-linolenic acid (ALA), 20:5ω3 eicosapentaenoic acid (EPA), 22:5ω3 docosapentaenoic acid (DPA), 22:6ω3 docosahexaenoic acid (DHA), 18:2ω6 linoleic acid (LA), 18:3ω6 γ-linolenic acid (GLA), 20:2ω6 eicosadienoic acid, 20:3ω6 dihomo-γ-linolenic acid (DGLA), 20:4ω6 arachidonic acid (ARA), 22:4ω6 adrenic acid, 22:5ω6 osbond acid (OsbA), 16:1ω7 palmitoleic acid, 18:1ω7 vaccenic acid, 18:1ω9 oleic acid, 20:1ω9 gondoic acid, 20:3ω9 mead acid, 24:1ω9 nervonic acid. Sat: Σsaturated FAs, MUFA: Σmono-unsaturated FAs, PUFA: Σpoly-unsaturated FAs. A-H: n = 9 (NL) and n = 5 (SL), Tukey box plots and scatter plots. Data represent the pair-housed PN70 cohort. Exact two-sided Mann-Whitey U test *: p<0.05, ** p<0.01, *** p<0.001.

Article Snippet: 11877771216), total cholesterol (TC, Roche, cat. no. 11491458–216), free cholesterol (FC, Spinreact, Santa Coloma, Spain, cat. no. 41035), non-esterified fatty acids (NEFA, Sopachem, 157819910935), phospholipids (Sopachem, Wageningen, the Netherlands, cat. no. 157419910930), aspartate transaminase (AST, Spinreact, cat. no. 1001165), and alanine transaminase (ALT, Spinreact, cat. no. 1001175).

Techniques: Clinical Proteomics, Gene Expression